Genotypic assay to determine some virulence factors of Uropathogenic E. coli (UPEC) isolates

A total of 179 urine samples were collected from patients suffering urinary tract infections admitted and visit Al-Hilla General Teaching Hospital in City, during a period April 2021 to December 2021, both sex (male female). Out 179,123 (68.7%) positive culture, whereas 56 (31.3%) showed no bacterial growth, To confirm the identification E. coli by use selective media (EMB agar medium, biochemical tests, automated Vitek 2 system 16s RNA specific primer presence (1492 bp) compared with allelic ladder, it was found that, deliberated main an etiological causes UTI other types bacteria which constitute 56/123 (45.5%), [45/56 (80.4%) female 11/56 (19.6%) male], while 67/123 (54.4%) related bacteria. Molecular detection some virulence factors genes studied, out isolates, hlyA gene detected 21/56 (37.5%) isolates (1177 sat 35/56 (62.5%) (410 ladder.